![]() With over 30 years of experience partnering with healthcare organizations, we understand the importance of continuing to serve you reliably, safely, and compliantly during the COVID-19 pandemic. We have made many strategic decisions related to collection, transportation, and disposal of regulated wastes to ensure our supply chain functions without disruption and to protect our customers from the financial burden of the pandemic. Understanding and correctly implementing the factors that lead to successful sterilization results in a significant achievement toward an overall effective dental infection control program.Our knowledge center is intended to keep you informed on the latest guidance for Coronavirus (COVID-19) waste disposal and help you prepare for the influx of waste generated by vaccinations. It also contains information on how we plan to continue service, including changes we are making to keep our employees, our customers and their patients, and our communities as safe as possible in the process. However, if you receive a positive spore test, contact your biological monitoring service immediately for assistance. In general, a single positive spore test probably does not indicate sterilizer malfunction, especially if the process indicators demonstrate sterilizer effectiveness. Also check the sterilizer for any obvious inconsistencies. Ensure that the process indicator has not expired and that the testing protocol has been met. If a process indicator turns positive, then retest with the process indicator immediately. They do not, however, prove that sterilization has occurred.Ĭommon factors for sterilization failures include chamber overload, excessive packaging material, inadequate exposure time, incorrect temperature/pressure settings, failure to preheat the sterilizer (if indicated), interruption of the sterilization cycle, and expired chemical solution (chemiclaves only). ![]() They designate that a package has been placed through a sterilization cycle. Process indicators such as autoclave tape are recommended within each load to show that certain physical parameters have been met within the sterilizer. Additionally, general sterilizer maintenance consisting of regular cleaning and checking of door gaskets, vents, and internal and external surfaces of the sterilizer should be conducted in accord with the sterilizer manufacturer’s instructions. It is good practice to place the strips in a different location of the sterilizer each week to help identify any “cold spots” within the sterilizer.Īll staff members who operate the sterilizer should receive training about proper sterilizer operation procedures and instrument processing techniques in the office. If there are no instructions, place the spore test strip within a wrapped set of instruments in the most difficult area to be sterilized, which is normally the lower front area of the sterilizer. The glassine-bound spore strips should be placed according to the sterilizer manufacturer’s instructions. Lot number and expiration date are printed on each glassine. This allows the tester to transport the strip within the glassine from the sterilizer to the processing area, where it can be properly handled. Glassine paper is permeable to sterilant but resistant to moisture and air at ambient temperature/pressure. Specific spores, Geobacillus stearothermophilus for steam/chemical sterilizers and Bacillus atrophaeus for dry heat sterilizers, are inoculated onto strips of specialized filter paper that is packaged in a peel open glassine paper pouch. When spores are killed during a sterilization cycle, it is assumed that all microorganisms have been destroyed and sterilization is achieved.īiological indicators consist of highly resistant, nonpathogenic bacterial spores. Regular sterilizer monitoring using biologic indicators has become the standard practice, and when properly implemented, is an important part of infection control verification.īoth the Centers for Disease Control and Prevention (CDC) and the American Dental Association recommend, and most state dental boards require, that dental offices verify the proper functioning of the sterilization cycle at least weekly using a biological indicator, such as the Spore Check System from OSHA Review, Inc. Monitoring of sterilization procedures includes a combination of process parameters to evaluate the sterilizing conditions and the procedures’ effectiveness.
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